Confocal optical microscope, magnifying attachment therefor...

G - Physics – 02 – B

Patent

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G02B 21/00 (2006.01)

Patent

CA 2348257

An optical attachment unit is described which can be inserted reversibly (31, 32) into the detection light path of a confocal optical microscope, which has the effect of modifying the magnification of the specimen at the level of the detector iris (36). This modification consists in the preferred form of a demagnification, so that high sensitivity is obtained at the expense of confocal function, as is required for multiphoton imaging and other purposes. No optical telescope (30) or other imaging means used for confocal function is disturbed or removed by the operation of inserting the unit, so that the confocal function can be restored reproducibly and rapidly.

L'invention concerne une unité optique fixable qui peut être insérée de manière réversible (31, 32) dans une voie de détection de lumière d'un microscope optique confocal, ce qui a pour effet de modifier l'agrandissement de l'échantillon au niveau de l'iris du détecteur (36). Dans un mode de réalisation préféré, la modification consiste en un grossissement inverse, ce qui permet d'obtenir une sensibilité élevée au prix de la fonction confocale, comme c'est requis pour l'imagerie à multiphotons ou à d'autres fins. L'opération d'insertion ne perturbe ni ne nécessite l'enlèvement d'aucune lunette optique (30) ni de tout autre système d'imagerie utilisé pour la fonction confocale; cela permet de restaurer la fonction confocale rapidement et de façon répétitive.

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