Conjugate for the detection and/or assay of a biological...

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/70 (2006.01) C12Q 1/68 (2006.01) G01N 33/535 (2006.01)

Patent

CA 2145092

Novel conjugate for the detection and/or assay of a biological compound, consisting of a kinase or a dehydrogenase, the sulphur groups being preferably re- duced beforehand, and capable of producing an inter- mediate compound for use in a bioluminescence sys- tem, preferably by means of a luciferase. Said enzyme is coupled to a ligand that was previously activated by a coupling agent and is capable of specifically bonding to said biological compound. The invention also con- cerns a process for the production of said conjugate, the application of said conjugate in detection and/or assay processes and a diagnostic kit containing same.

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