Conjugate purification

A - Human Necessities – 61 – K

Patent

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Details

A61K 47/48 (2006.01) A61K 39/09 (2006.01) A61P 31/04 (2006.01) B01D 15/00 (2006.01) B01J 20/04 (2006.01)

Patent

CA 2693936

This application relates to methods for the purification of saccharide antigen- carrier protein conjugates. In particu-lar, the invention provides a method for purifying saccharide antigen- carrier protein conjugates from free carrier protein, such as CRM1 97, using hydroxyapatite. The invention further relates to methods of preparing vaccines, using this method.

L'invention concerne des procédés de purification de conjugués antigène saccharidique-protéine de support. L'invention concerne en particulier un procédé de purification des conjugués antigène saccharidique-protéine de support d'une protéine de support libre, par exemple CRMl 97, à l'aide d'hydroxyapatite. L'invention concerne également des méthodes de préparation de vaccins à l'aide dudit procédé.

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