Conjugate vaccine against infections by gram-negative...

A - Human Necessities – 61 – K

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A61K 39/02 (2006.01) A61K 39/104 (2006.01) C07K 16/12 (2006.01)

Patent

CA 1276552

Abstract of the disclosure: The invention relates to a non-toxic conjugate vaccine against infections by Gram-negative bacteria, composed of a species-specific polysaccharide and a protein which are covalently linked together, it being characteristic of the vaccine that the polysaccharide originates from the endotoxin of the bacterium and the protein is an exoprotein. The vaccines according to the invention are, in particular, vaccines against infections by Pseudomonas aeruginosa or Escherichia coli. The exoprotein is, in particular, an exotoxin or exotoxoid, preferably toxin A from Pseudomonas aeru- ginosa, but also a tetanus toxoid or diphtheria toxoid. The process for the preparation of the new con- jugate vaccine against infections by Gram-negative bacteria comprises covalent linkage of an appropriate polysaccharide from the endotoxin of the bacterium with an exoprotein. However, the invention also relates to a con- jugate vaccine composed of a mixture of 3-15 conjugates whose polysaccharide have (sic) been obtained from 3-15 different serotypes of the same species of bacteria. The invention further relates to a polyvalent vaccine composed of a mixture of conjugate vaccines, each of whose individual components raises antibodies against a particular species of bacteria. The invention further relates to the use of the new conjugate vaccines for the preparation of hyper- immune sera which in turn are used for the preparation of immunoglobulin which can be administered intra- venously or intramuscularly.

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