Conjugation of c-myc antisense oligonucleotides with...

A - Human Necessities – 61 – K

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A61K 47/48 (2006.01) C12N 15/11 (2006.01) A61K 38/00 (2006.01)

Patent

CA 2241835

Antisense oligonucleotides have recently been proposed as a pharmacological approach to prevent neointimal hyperplasia following balloon angioplasty. This study reports the significant enhancement on in vivo restenosis prevention conferred by the conjugation of cholesterol to a c-myc antisense oligomer when compared to a non conjugated oligonucleotide. The inhibitory potential of the conjugate was increased 2.4-fold, reaching a reduction of intimal hyperplasia of 84.7 % vs. control arteries. Enhancement of hyperplasia prevention may be related to the 2.7-fold increase in oligomer vascular retention conferred by the cholesterol moieties, sufficient to cover a significant portion of the proliferation window that typically follows balloon angioplasty.

Les oligonucléotides anti-sens ont été récemment proposés comme une voie pharmacologique pour prévenir l'hyperplasie de la néo-intima consécutive à l'angioplastie par ballonnet. La présente étude rapporte le renforcement important obtenu, pour la prévention de la resténose in vivo, par la conjugaison de cholesterol à un oligomère anti-sens c-myc par rapport à un oligonucleotide non conjugué. Le potentiel inhibiteur du conjugué a été multiplié par 2,4, ce qui a permis d'obtenir une réduction de 84,7 % de l'hyperplasie de l'intima par rapport aux artères témoins. Le renforcement de la prévention de l'hyperplasie peut être mis en relation avec la multiplication par 2,7 de la rétention vasculaire d'oligomères produite par les fragments de cholesterol, qui est suffisante pour couvrir une partie importante de la fenêtre de prolifération qui est caractéristique de ce qui suit l'angioplastie par ballonnet.

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