Conjugation process

A - Human Necessities – 61 – K

Patent

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Details

A61K 47/48 (2006.01) A61K 39/085 (2006.01) A61K 39/385 (2006.01) A61P 31/04 (2006.01)

Patent

CA 2647455

The present application describes a process for conjugating a PNAG which is less than 40% N-acetylated to a carrier protein comprising the steps of a) activating the PNAG by adding a linker comprising a maleimide group to form an activated PNAG b) activating the carrier protein by adding a linker comprising a sulphydryl group to form an activated carrier protein and c) reacting the activated PNAG and the activated carrier protein to form a PNAG-carrier protein conjugate.

L'invention concerne un procédé de conjugaison d'un PNAG N-acétylé à moins de 40% à une protéine porteuse, consistant a) à activer le PNAG par addition d'un agent de liaison comprenant un groupe maléimide pour former un PNAG activé, b) à activer la protéine porteuse par addition d'un agent de liaison comprenant un groupe sulphydryle pour former une protéine porteuse activée, et c) à faire réagir le PNAG activé et la protéine porteuse activée pour former un conjugué PNAG-protéine porteuse.

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