Conservative whole blood sample preparation technique

Details Conservative whole blood sample preparation technique Conservative whole blood sample preparation technique

G - Physics

01

N

150/11, 167/322

G01N 1/28 (2006.01) A01N 1/02 (2006.01) G01N 1/30 (2006.01) G01N 33/50 (2006.01) G01N 33/569 (2006.01)

Patent

CA 1319592

ABSTRACT A method for the rapid preparation of a whole blood sample for photooptical analysis. A whole blood sample, lytic reagent system and stain are contacted in a reaction vessel with gentle, asymmetric vortex mixing. This method conserves the endogenous constituents of the sample, since the preparation of the sample occurs within a common vessel and any excess, unconsumed or unreacted, reagents and stain are not separated from the sample during preparation, thus avoiding the multiple wash steps traditionally employed. The lytic agent preferably is acidic and stromatolyzes the erythrocyte fraction of the whole blood, leaving the leukocyte sub-populations in an unlysed, near native state. The lysing can occur, before, simultaneous with, or after the staining in an elapsed time of 90 seconds or less.

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