Constellation mapping and uses thereof

Details Constellation mapping and uses thereof Constellation mapping and uses thereof

G - Physics

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G01N 33/68 (2006.01) G01N 30/86 (2006.01) G06F 19/00 (2006.01) H01J 49/00 (2006.01) G01N 30/72 (2006.01)

Patent

CA 2503292

The present invention features computer methods and systems for comparing biomolecules across biological samples. In these methods, mass spectrometry measurements are obtained on biomolecules in two or more samples. These measurements are then processed and analyzed by the methods described herein to render them more comparable. We refer to this technology as ~Constellation Mapping~ (CM). The resulting data, constellation maps, can be used to compare the abundance of biomolecules across samples, and, when done in real time, can be used to select differentially abundant biomolecules for subsequent LC/MS-MS.

La présente invention concerne des procédés et des systèmes informatiques utilisés pour comparer des biomolécules entre échantillons biologiques. Dans ce contexte, on effectue des relevés de spectrométrie de masse sur des biomolécules prises dans deux échantillons ou plus. Ces relevés sont ensuite traités et analysés au moyen des méthodes décrites à des fins de comparabilité. Cette technologie est dite de <= mise en correspondance de constellations >=/Constellation Mapping (CM). Les résultats ainsi obtenus, ou cartes de constellation, peuvent servir à comparer la quantité 'abondance de biomolécules dans des échantillons et, en temps réel, être utilisés pour la sélection de biomolécules en abondance variable pour LC/MS-MS ultérieures (chromatiographie liquide/spectrométrie de masse en tandem).

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