Constitutive promoters from poplar and uses thereof

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/82 (2006.01) A01H 5/00 (2006.01) C12N 5/04 (2006.01) C12N 5/10 (2006.01) C12N 15/11 (2006.01) C12P 21/02 (2006.01)

Patent

CA 2614361

The present invention relates to nucleic acid molecules corresponding to regulatory portions of genes whose expression is constitutive. The invention also relates to compositions and methods of using the same to regulate the expression, in a constitutive manner, of genes and/or any kind of nucleotide sequences in a plant. Nucleic acid molecules and its compositions include novel nucleotide sequences for constitutive promoter identified in and isolated from poplar (Populus spp). Methods for expressing genes and/or any kind of nucleotide sequences in a plant using the promoter sequences disclosed herein are provided. The methods comprise stably incorporating into the genome of a plant cell a nucleotide sequence operably linked to one or more of the constitutive promoters of the present invention and regenerating a stably transformed plant that expresses the nucleotide sequence.

L'invention concerne des molécules d'acides nucléiques correspondant à des parties régulatrices de gènes dont l'expression est constitutive. L'invention concerne également des compositions et procédés d'utilisation de ces molécules pour réguler l'expression, de façon constitutive, de gènes et/ou de n'importe quelle sorte de séquences nucléotidiques dans une plante. Les molécules d'acides nucléiques et les compositions les contenant comprennent de nouvelles séquences nucléotidiques pour un promoteur constitutif identifié et isolé du peuplier (Populus spp). L'invention concerne encore des procédés d'expression de gènes et/ou de n'importe quelle autre sorte de séquences nucléotidiques dans une plante au moyen des séquences de promoteurs de cette invention. Ces procédés consistent à incorporer de façon stable dans le génome d'une cellule de plante une séquence nucléotidique liée dans son fonctionnement à un ou plusieurs promoteurs constitutifs de cette invention, puis à régénérer une plante transformée de façon stable et qui exprime cette séquence nucléotidique.

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