Construction of an igg binding protein to facilitate...

Details Construction of an igg binding protein to facilitate... Construction of an igg binding protein to facilitate...

C - Chemistry, Metallurgy

12

N

530/13, 530/3.08

C12N 15/62 (2006.01) C07K 14/31 (2006.01) C07K 14/65 (2006.01) C12N 1/21 (2006.01) C12N 15/66 (2006.01)

Patent

CA 1311430

ABSTRACT A recombinant DNA fragment (Z) coding for an immuno- globulin G binding domain related to staphylococcal protein A, characterized in that the methionine codon of said fragment has been replaced by a codon of another amino acid residue enabling expression of a methionine- free protein; a recombinant DNA sequence comprising at least two such fragments; a recombinant DNA sequence comprising such fragment preceded by a signal sequence and followed by a nucleotide sequence coding for the amino acid se- quence: Ala Gln His Asp Glu Ala; a recombinant DNA molecule comprising such recombinant DNA sequence and fused 3' thereof at DNA level a pro- duction gene, said molecule having the ability to express a fused protein; a process for cleaving a fused protein expressed in a biological system by a such recombinant DNA molecule; a plasmid vector comprising said recombinant DNA molecule; and a bacterial cell harbouring such recombinant DNA molecule.

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