Construction of barley with decreased gel protein content

A - Human Necessities – 01 – H

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A01H 5/00 (2006.01) C07K 14/415 (2006.01) C12N 15/10 (2006.01) C12N 15/63 (2006.01) C12N 15/82 (2006.01)

Patent

CA 2379259

The amount of D-hordein, which constitutes gel proteins, formed in barley is decreased. The decrease in the D-hordein yield causes not only a decrease in the gel proteins due to the decrease in the D-hordein fraction in the gel proteins but also the migration (decrease) of proteins other than D-hordein, which constitute the gel proteins, from the gel proteins. Accordingly, a decrease in the D-hordein yield in barley and, in its turn, a decrease in the gel proteins can be achieved by transferring a D-hordein expression regulatory vector 1, whereby the D-hordein yield can be regulated, into barley to thereby transform the barley.

Selon l'invention, la quantité de D-hordéine, qui constitue les protéines gels, formée dans l'orge est réduite. La réduction du rendement de D-hordéine entraîne non seulement une réduction des protéines gels due à la réduction de la fraction de D-hordéine dans les protéines gels, mais également la migration (réduction) des protéines autres que la D-hordéine, qui constituent les protéines gels, à partir des protéines gels. En conséquence, une réduction du rendement de D-hordéine dans l'orge et une réduction des protéines gels peuvent être obtenues par transfert d'un vecteur 1 régulateur de l'expression de D-hordéine, permettant la régulation du rendement de D-hordéine, dans l'orge, pour ainsi transformer cette dernière.

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