Construction of highly efficient cellulase compositions for...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/56 (2006.01) C12N 1/14 (2006.01) C12N 1/15 (2006.01) C12N 9/24 (2006.01) C12N 9/42 (2006.01) C12P 1/00 (2006.01) C12P 7/10 (2006.01) C12P 19/00 (2006.01) C12P 19/02 (2006.01) C12P 19/04 (2006.01)

Patent

CA 2657684

This invention provides novel enzyme compositions using newly identified and isolated C. lucknowense enzymes, including CBH Ib, CBH IIb, EG II, EG VI, .beta.-glucosidase, and xylanase II in conjunction with previously identified enzymes CBH Ia, CBH IIa (previously described as Endo 43), and EG V. These enzyme compositions demonstrate an extremely high ability to convert lignocellulosic biomass (e.g., Avicel, cotton, Douglas fir wood pretreated by organosolv) to glucose. CBH Ia and IIb, which both have a cellulose-binding module (CBM) displayed a pronounced synergism with three major endoglucanases (EG II, EG V, EG VI) from the same fungus in hydrolysis of cotton as well as a strong synergy with each other. The enzyme compositions are effective in hydrolysis of the lignocellulosic biomass.

Cette invention concerne de nouvelles compositions enzymatiques utilisant des enzymes de C. lucknowense nouvellement identifiées et isolées, comprenant CBH Ib, CBH IIb, EG II, EG VI, la .beta.-glucosidase et la xylanase II, conjointement avec les enzymes précédemment identifiées CBH Ia, CBH IIa (précédemment décrites comme étant Endo 43) et EG V. Ces compositions enzymatiques démontrent une aptitude extrêmement élevée à convertir la biomasse lignocellulosique (par exemple, Avicel, le coton, le bois de sapin de Douglas pré-traité par un solvant organique) en glucose. CBH Ia et IIb, qui ont toutes deux un module de liaison à la cellulose (CBM), ont présenté une synergie prononcée avec trois endoglucanases majeures (EG II, EG V, EG VI) provenant du même champignon dans l'hydrolyse du coton, ainsi qu'une forte synergie l'une avec l'autre. Les compositions enzymatiques sont efficaces dans l'hydrolyse de la biomasse lignocellulosique.

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