Construction of new .alpha.-galactosidase producing yeast...

Details Construction of new .alpha.-galactosidase producing yeast... Construction of new .alpha.-galactosidase producing yeast...

C - Chemistry, Metallurgy

12

N

195/55, 150/15.2

C12N 15/56 (2006.01) C12C 11/00 (2006.01) C12N 1/18 (2006.01) C12N 1/19 (2006.01) C12N 9/40 (2006.01) C12N 15/10 (2006.01) C12N 15/81 (2006.01) C12N 15/90 (2006.01) C12Q 1/34 (2006.01) G01N 33/14 (2006.01)

Patent

CA 1334511

New Saccharomyces cerevisiae yeast strains having .alpha.- galactosidase gene (MEL+) transferred by recombinant DNA methods are disclosed. The new strains produce more .alpha.-galactosidase than naturally .alpha.-galactosidase producing yeast strains. Methods for marking yeast strains and for producing stable transformants of yeasts are also disclosed. Baker's and distiller's yeasts producing .alpha.-galactosidase are used in the corresponding industries, due to their ability to utilize the raffinose present in molasses, which results in greater yield of yeast (or ethanol) and reduction or elimination of the costs associated with biological oxygen demand (B.O.D.) in the effluent from factories. The improved ability of brewer's yeasts to produce .alpha.-galactosidase provides a sensitive method for monitoring pasteurization of beer.

534397

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