Constructs and methods for expression of recombinant hcv...

C - Chemistry – Metallurgy – 12 – N

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C12N 1/16 (2006.01) C07K 14/18 (2006.01) C12N 7/04 (2006.01) A61K 38/00 (2006.01) A61K 39/00 (2006.01)

Patent

CA 2443740

The current invention relates to vectors and methods for efficient expression of HCV envelope proteins in eukaryotic cells. More particularly said vectors comprise the coding sequence for an avian lysozyme signal peptide or a functional equivalent thereof joined to a HCV envelope protein or a part thereof. Said avian lysozyme signal peptide is efficiently removed when the protein comprising said avian lysozyme signal peptide joined to a HCV envelope protein or a part thereof is expressed in a eukaryotic cell. Suitable eukaryotic cells include yeast cells such as Saccharomyces or Hansenula cells.

La présente invention concerne des vecteurs et des méthodes permettant une expression efficace de protéines d'enveloppe du VHC dans des cellules eucaryotes. Plus particulièrement, lesdits vecteurs comprennent la séquence codante pour un peptide signal du lysozyme aviaire ou un équivalent fonctionnel dudit peptide, lié à une protéine d'enveloppe du VHC ou une partie de ladite protéine. Ce peptide signal du lysozyme aviaire est retiré, de façon efficace, lorsque la protéine renfermant ledit peptide signal du lysozyme aviaire, lié à une protéine d'enveloppe du VHC ou une partie de cette dernière, est exprimée dans une cellule eucaryote. Parmi les cellules eucaryotes adaptées figurent des cellules de levure, telles que les cellules de Saccharomyces ou Hansenula.

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