Constructs and methods for the production and secretion of...

C - Chemistry – Metallurgy – 12 – N

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C12N 9/42 (2006.01) C12N 15/74 (2006.01) C12P 21/02 (2006.01) C12N 15/62 (2006.01) C12P 7/10 (2006.01)

Patent

CA 2731203

Described herein are molecules, constructs and methods for the production and secretion of polypeptides of interest by host cells, preferably bacterial host cells, and more particularly gram positive bacteria. In particular, the present invention is related to a polynucleic acid encoding a fusion protein and to uses thereof for the secretion of heterologous or homologous polypeptides of interest by a bacterial host cell, preferably Clostridium bacteria. The present invention further relates to methods and constructs for the production and secretion of heterologous or homologous polypeptides of interest proteins by host cells using such polynucleic acids and fusion proteins.

La présente invention concerne des molécules, des constructions et des procédés de production et de sécrétion de polypeptides d'intérêt par des cellules hôtes, de préférence des cellules hôtes bactériennes, et plus particulièrement des bactéries à Gram positif. En particulier, la présente invention porte sur un acide polynucléique codant pour une protéine de fusion, ainsi que sur des utilisations de celui-ci pour la sécrétion de polypeptides d'intérêt hétérologues ou homologues par une cellule hôte bactérienne, de préférence la bactérie Clostridium. La présente invention concerne en outre des procédés et des constructions de production et de sécrétion de polypeptides hétérologues ou homologues de protéines d'intérêt par des cellules hôtes utilisant de tels acides polynucléiques et des protéines de fusion.

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