Containers for culturing and testing of vertebrate lenses

C - Chemistry – Metallurgy – 12 – M

Patent

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C12M 1/16 (2006.01) C12M 3/00 (2006.01)

Patent

CA 1236788

A B S T R A C T A container for holding and culturing a lens is disclosed 7 comprising a transparent base member, a transparent lens carrier supported laterally in the base member and having a central aperture, and a transparent cover engaging the base member for enclosing the lens carrier within the container. The base is circular in top view, and in sectional elevation is in the form of a flanged U-shape, forming a well. The lens carrier is a circular plate with a central aperture. The lip of the aperture tapers downwardly in order to support the outer rim of the lens to be tested. The cover has an annular flange and a raised centre section with angled sidewalls. The sidewalls have two flat sections offset from each other by 90 degrees, which act as viewing ports. The lens to be studied is centered in the aperture in the bottom of the lens carrier. The lens carrier is ordinarily provided with passage holes to permit the free passage of culture medium from one side of the lens to the other. However, a lens carrier without such holes may be used if it is desired to expose the anterior and posterior lens surfaces to separate media.

479406

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