Continuous culture apparatus with mobile vessel, allowing...

C - Chemistry – Metallurgy – 12 – M

Patent

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Details

C12M 1/36 (2006.01)

Patent

CA 2557574

Method and device that increases the rate of reproduction (through increased speed of reproduction and/or increased reproductive yield) of living cells in suspension or of any culturable organisms through the process of natural selection, said device comprising: a) a flexible, sterile tube (7) containing culture medium, b) a system of movable gates (clamps) (3, 4, 5) that divide the tube (97) into separate regions containing spent culture (downstream region), growing culture (growth chamber), and fresh growth medium (upstream region), c) a means of moving the gates and the tubing (13) such that a portion of the growth chamber and the associated culture can be clamped off and separated from the growth chamber, and such that a portion of fresh tubing containing unused medium can be joined with a portion of the culture and associated medium already present in the growth chamber.

L'invention concerne un procédé et un dispositif permettant d'augmenter le débit de reproduction (par le biais d'une vitesse accrue de reproduction et/ou d'un rendement de reproduction accru) de cellules vivantes en suspension ou de quelconques organismes cultivables par le biais du processus de sélection naturelle. Ledit dispositif comprend (a) un tuyau flexible, stérile (7) contenant un milieu de culture, (b) un système de portes mobiles (fixations) (3, 4, 5) qui divisent le tuyau (97) en zones séparées renfermant la culture usagée (zone en aval), la culture croissante (chambre de croissance), et le milieu de culture frais (zone en amont), et (c) un dispositif conçu pour déplacer les portes et les tuyaux (13) de telle manière qu'une partie de la chambre de croissance et la culture associée peuvent être détachées et séparées de la chambre de croissance et qu'une partie des tuyaux frais contenant le milieu non utilisé peut être jointe à une partie de la culture et au milieu associé déjà présent dans la chambre de croissance.

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