Continuous-flow method for preparing microparticles

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/10 (2006.01) A61K 9/16 (2006.01) A61K 31/7088 (2006.01) A61K 47/34 (2006.01) A61K 48/00 (2006.01) C12M 1/00 (2006.01) C12N 15/64 (2006.01) C12N 15/87 (2006.01)

Patent

CA 2389613

The invention is based on the discovery of a method for scalable, continuous flow production of a nucleic acid-containing microparticle that maintains the structural integrity of the associated nucleic acid and results in a microparticle having a purity suitable for introduction into an animal (e.g., human) host. Microparticles prepared according to the continuous flow processes described herein can be used for delivery of a nucleic acid for gene therapy, antisense therapy, vaccination, treatment of autoimmune disease, and either specific or non-specific modulation of an immune response (e.g., via cytokine regulation). The microparticles can additionally be used to deliver nucleic acid encoding a protein or peptide useful in any type of therapy.

Cette invention a trait à un procédé évolutif, de production à débit continu de microparticules contenant de l'acide nucléique, lequel procédé conserve à l'acide nucléique son intégrité structurelle et permet d'obtenir des microparticules dont la pureté se prête à une administration à un hôte, à un être humain notamment. Il est possible d'utiliser les microparticules produites grâce à ce procédé à débit continu aux fins de l'administration d'acide nucléique dans le cadre d'une thérapie génique, d'une thérapie antisens, d'une vaccination, du traitement d'une maladie auto-immune ainsi que dans le cadre d'une modulation spécifique ou non spécifique d'une réponse immunitaire (par exemple, par le biais d'une régulation de la cytokine). Il est, en outre, possible d'utiliser ces microparticules pour administrer un acide nucléique codant une protéine ou un peptide utilisable dans n'importe quel type de thérapie.

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