C - Chemistry – Metallurgy – 12 – P
Patent
C - Chemistry, Metallurgy
12
P
195/140
C12P 19/04 (2006.01) C12R 1/05 (1980.01)
Patent
CA 1158580
A CONTINUOUS PROCESS FOR THE PRODUCTION OF GELABLE EXOPOLYSACCHARIDE Abstract of the Disclosure The present invention is directed to a two-stage continuous process for the production of a gelable curdlan- type exopolysaccharide. In the first stage a stable, curdlan- producing strain of microorganism such as Alcaligenes faecalis var. myxogenes ATCC 31749 and ATCC 31750, is grown aerobically in an aerated, agitated culture medium containing assimilable carbon, nutrients and organic salts. The amount of nitrogen in the first stage is so limited that the effluent therefrom contains substantially no inorganic nitrogen. The effluent is introduced into a second stage in a constant volume fermenter wherein it is mixed with a nitrogen-free carbohy- drate. The resultant mixture is aerated and mixed at pH 5.5 to 6.5 at a temperature of from 25 to 35°C, the volume and dilution rate in the reactor being selected so that the residence time of the microorganism, in the fermenter does not exceed an equivalent to the maximum length of time during which the activity of a batch culture of the microorganism with respect to product synthesis is maximal. The product is subsequently isolated.
393724
Bereskin & Parr
Weston (george) Limited
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