Control of male fertility using externally inducible...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/82 (2006.01) A01H 1/02 (2006.01) A01H 5/10 (2006.01) C12N 5/04 (2006.01) C12N 9/02 (2006.01)

Patent

CA 2131819

An inducible promoter is used to regulate expression of a gene which is known to be critical to male fertility. The selected gene's control sequences are modified so that it is normally "off" and as a result the plants are male sterile. When it is desired to reproduce the male sterile plants, male fertility is restored by treating the plants with a non-phytotoxic chemical which induces expression of the critical gene. A critical gene is one which affects flavonol production and in particular compounds of formula (1), wherein R1, R2, R3, R4, R5, R7, and R8 are hydrogen, hydroxyl or alkoxy having from 1 to 4 carbon atoms. Particularly preferred flavonols include galangin, kaempferol, iso-rhamnetin, quercetin and morin.

Un promoteur inductible est utilisé pour réguler l'expression d'un gène qui est connu comme déterminant pour la fertilité mâle. Les séquences de régulation du gène sont modifiées de manière que le gène soit normalement en position désactivée et, en conséquence, les plantes sont mâles stériles. Lorsqu'on désire provoquer la reproduction des plantes mâles stériles, on rétablit la fertilité mâle en traitant les plantes avec un produit chimique non phytotoxique qui induit l'expression du gène déterminant. Un gène déterminant est un gène qui affecte la production de flavonol et particulièrement des composés de formule (I), où R1, R2, R3, R4, R5, R7 et R8 sont un hydrogène, un hydroxyle ou un alcoxy de 1 à 4 atomes de carbone. Parmi les flavonols que l'on juge plus particulièrement utiles figurent la galangine, le kaempférol, l'isorhamnétine, la quercétine et la morine.

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