Control of ph during bordetella growth

C - Chemistry – Metallurgy – 12 – N

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C12N 1/20 (2006.01) A61K 39/10 (2006.01)

Patent

CA 2138178

Bordetella species, such as Bordetella pertussis, is grown under aerobic conditions in a liquid culture medium containing nutrients for such growth. Enhanced cell density and antigen production are achieved by continuously determining the pH of the culture medium and, in response to such determination, simultaneously feeding, as required, (a) an acid, usually a mineral acid, such as hydrochloric acid, phosphoric acid or sulfuric acid, in a concentration sufficient to maintain the pH of the culture medium within a predetermined range of values, usually about pH 6.8 to about pH 7.3, and (b) a nutrient medium containing organic carbon and organic nitrogen, usually a neutral salt of an amino acid, such as monosodium glutamate, an amino acid, such as glutamic acid, or a mixture thereof.

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