Control of temperature gradients during gel electrophoresis...

G - Physics – 01 – N

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G01N 27/447 (2006.01)

Patent

CA 2187097

A gel electrophoresis separation apparatus comprising, in combination, a gaseous heat-exchange medium, a gaseous heat-exchange medium driving means, and an impingement means, whereby the gaseous heat-exhange medium is driven by the gaseous heat-exchange medium driving means across the impingement means to provide a flow of the gaseous heat-exchange medium on the surface of the gel plates, whereby the flow induced by passage of the gaseous heat-exchange medium through the impingement means thereby minimizes temperature gradients within the gel by forced convection.

La présente invention concerne un dispositif de séparation par électrophorèse sur gel. Dans le dispositif, on utilise, en combinaison, un gaz d'échange thermique, un organe d'entraînement du gaz d'échange thermique, et un dispositif à points de soufflage. L'organe d'entraînement du gaz d'échange thermique fait ainsi passer le gaz d'échange thermique au travers du dispositif à points de soufflage, ce qui crée un flux de gaz d'échange thermique sur les plaques de gel. Le flux induit par le passage du gaz d'échange thermique au travers du dispositif à points de soufflage réduit ainsi au minimum, par convection forcée, les gradients thermiques à l'intérieur du gel.

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