Controlled excision

Details Controlled excision Controlled excision

C - Chemistry, Metallurgy

12

N

195/134, 195/1.1

C12N 1/21 (2006.01) C12N 15/52 (2006.01) C12N 15/67 (2006.01) C12N 15/73 (2006.01) C12N 15/90 (2006.01) C12P 13/04 (2006.01) C12P 13/22 (2006.01)

Patent

CA 1339799

Integrating a gene in a bacterial chromosome and controlling excision of the gene from the chromosome in response to an external stimulus, using DNA comprising: a) a vector that is incapable of replicating autonomously in the host and that includes the gene (or a site for the insertion of the gene) and an attachment site corresponding to an attachment site on the host chromosome; b) DNA capable of causing the integration of vector a) in the bacterial chromosome and DNA capable of causing the excision of that vector therefrom; and c) DNA capable of effecting control of excision of vector a) from the bacterial chromosome in response to an externally controlled stimulus.

501705

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