C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/10 (2006.01) C12Q 1/68 (2006.01) C12Q 1/70 (2006.01)
Patent
CA 2049879
Abstract of the Disclosure An improvement to the ligase chain reaction (LCR) method of selectively and geometrically ligating nucleic acid probes; before the LCR, a template-dependent controlled reaction increases the population of templates capable of supporting template-dependent ligation in LCR selectively in the presence of target nucleic acid, while substantially avoiding creation of any molecules capable of supporting template-dependent ligation in the absence of target nucleic acid. One way to control the initial reaction is to weight the reaction to create templates corresponding to only one of the two target strands, using e.g. template dependent ligation or polymerization. Another way to control the initial amplification is to create templates corresponding to both strands, but to prevent spurious creation of ligation templates by using a probe concentration low enough to avoid blunt end ligation or by using a method of amplification such as PCR that does not generate spurious ligation templates. 23
Backman Keith C.
Carrino John J.
Shimer George H.
Abbott Laboratories
Backman Keith C.
Biotechnica International Inc. To Abbott Laboratories
Biotechnica International Inc.; Abbott Laboratories
Carrino John J.
LandOfFree
Controlled initial target-dependent production of templates... does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Controlled initial target-dependent production of templates..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Controlled initial target-dependent production of templates... will most certainly appreciate the feedback.
Profile ID: LFCA-PAI-O-1781640