Controlled initiation of primer extension

Details Controlled initiation of primer extension Controlled initiation of primer extension

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01) C12P 19/34 (2006.01)

Patent

CA 2655018

Controlled initiation of primer extension in determination of nucleic acid sequence information by incorporation of nucleotides or nucleotide analogs. Preferred aspects include photo-initiated extension through the use of photo-cleavable blocking groups on termini of primer sequences followed by non-terminating primer extension using nucleotides or nucleotide analogs that are not extension terminators.

La présente invention concerne l'initiation régulée de l'extension d'amorce pour la détermination d'informations relatives à des séquences d'acides nucléiques par l'incorporation de nucléotides ou d'analogues de nucléotides. Les aspects préférés de l'invention incluent l'extension photo-initiée grâce à l'utilisation de groupes de blocage photo-clivables sur l'extrémité terminale de séquences d'amorce suivie d'une extension d'amorce non terminale au moyen de nucléotides ou d'analogues de nucléotides qui ne sont pas des terminateurs d'extension.

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