Core-glycosylated hcv envelope proteins

Details Core-glycosylated hcv envelope proteins Core-glycosylated hcv envelope proteins

C - Chemistry, Metallurgy

07

K

C07K 14/18 (2006.01) A61K 39/29 (2006.01) A61P 31/14 (2006.01) C12N 1/16 (2006.01) C12N 7/04 (2006.01) C12P 21/02 (2006.01) G01N 33/569 (2006.01) A61K 38/00 (2006.01) A61K 39/00 (2006.01)

Patent

CA 2443781

The current invention relates to HCV envelope proteins or parts thereof which are the product of expression in eukaryotic cells. More particularly said HCV envelope proteins are characterized in that on average up to 80 % of their N- glycosylation sites are core-glycosylated. Of these N-glycosylated sites more than 70 % are glycosylated with an oligomannose containing 8 to 10 mannoses. Furthermore, the ratio of the oligomannoses with structure Man(7)-GlcNAc(2) over the oligomannose with structure Man(8)-GlcNAc(2) is less than or equal to 0.45. Less than 10 % of the oligomannoses is terminated with an .alpha.1,3 linked mannose. The HCV envelope proteins of the invention are particularly suited for diagnostic, prophylactic and therapeutic purposes. A suitable eukaryotic cell for production of the HCV envelope proteins of the invention is a Hansenula cell.

L'invention porte sur des protéines d'enveloppe VHC ou des parties d'enveloppe VHC qui résultent de l'expression des cellules eucaryotes. Plus précisément, ces protéines d'enveloppe VHC se caractérisent par le fait que, en moyenne, jusqu'à 80 % de leurs sites de N-glycosylation sont glycolsylés au centre. Sur ces sites glycosylés au centre, plus de 70 % sont glycosylés avec une oligomannose possédant une structure définie par Man(8 à 10)-GlcNAc(2). Par ailleurs, le rapport oligomannose de structure Man(7)-GlcNAc(2) oligomannose de structure Man(8 à 10)-GlcNAc(2) est inférieur ou égal à 0,45. Moins de 10 % des oligomannoses se terminent par un mannose lié à .alpha.1,3. Les protéines d'enveloppe VHC selon l'invention conviennent tout particulièrement à des fins diagnostiques, prophylactiques et thérapeutiques. Une cellule eucaryote utile à la fabrication de protéines d'enveloppe VHC est une celluleHansenula.

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