Corneal epithelial sheet, method of constructing the same...

Details Corneal epithelial sheet, method of constructing the same... Corneal epithelial sheet, method of constructing the same...

A - Human Necessities

61

L

A61L 27/00 (2006.01) C12N 5/06 (2006.01)

Patent

CA 2559582

It is intended to provide a corneal epithelial sheet having potential for the achievement of a favorable therapeutic effect and being highly safe in transplantation. Namely, a corneal epithelial sheet is constructed by: (a) preparing corneal epithelial cells; (b) separately culturing human fibroblasts in a collagen gel; (c) sowing or placing the corneal epithelial cells on the collagen gel; and (d) culturing and proliferating the corneal epithelial cells in the absence of heterologous animal cells.

Il est prévu de fournir une feuille épithéliale de cornée ayant le potentiel de parvenir à un effet thérapeutique favorable et d'être très sûre pour la transplantation. A savoir que l'on crée une feuille épithéliale de cornée en : (a) préparant les cellules épithéliales de cornée, (b) cultivant séparément les fibroblastes humains dans un gel de collagène, (c) semant ou plaçant les cellules épithéliales de cornée sur le gel de collagène et en (d) cultivant et faisant proliférer les cellules épithéliales de cornée en l'absence de cellules animales xénogéniques.

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