Coryneform bacterium transformant and process for producing...

Details Coryneform bacterium transformant and process for producing... Coryneform bacterium transformant and process for producing...

C - Chemistry, Metallurgy

12

N

C12N 1/21 (2006.01) C12N 15/09 (2006.01) C12P 7/16 (2006.01)

Patent

CA 2755310

A Corynebacterium glutamicum transformant having the capability of producing isobutanol and the following genes (1) to (5): (1) a gene which encodes an enzyme having acetohydroxy acid synthase activity; (2) a gene which encodes an enzyme having acetohydroxy acid isomeroreductase activity; (3) a gene which encodes an enzyme having dihydroxy acid dehydratase activity; (4) a gene which encodes an enzyme having 2-keto acid decarboxylase activity; and (5) a gene which encodes an enzyme having alcohol dehydrogenase activity, at least one of the genes being endogenous, and at least one of the genes being exogenous, efficiently produces isobutanol.

L'invention porte sur un transformant capable de produire de l'isobutanol, qui est caractérisé en ce qu'il comprend Corynebacterium glutamicum ayant les gènes (1) à (5) mentionnés ci-après, au moins l'un des gènes (1) à (5) étant un gène endogène de Corynebacterium glutamicum et au moins l'un des gènes (1) à (5) étant un gène étranger : (1) un gène codant pour une enzyme ayant une activité acétohydroxy acide synthase ; (2) un gène codant pour une enzyme ayant une activité acétohydroxy acide isoméroréductase ; (3) un gène codant pour une enzyme ayant une activité dihydroxy acide déhydratase ; (4) un gène codant pour une enzyme ayant une activité 2-céto acide décarboxylase ; et (5) un gène codant pour une enzyme ayant une activité alcool déshydrogénase.

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