Coumarin-based cyanine dyes for non-specific protein binding

G - Physics – 01 – N

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G01N 33/00 (2006.01) C07F 9/24 (2006.01)

Patent

CA 2592159

Protein dyes whose molecular structure is that of a coumarin moiety coupled to a quaternary ammonium heterocycle through a vinyl or polyvinyl linkage demonstrate the ability to associate with proteins in a non-covalent, non- specific manner at low pH, where the associated form displays a significantly higher fluorescence emission than the unassociated form. This makes the dyes useful as selective labels for proteins at the low pH and eliminates the need for the removal of extraneous components from the medium in which the proteins reside prior to detection.

L'invention porte sur des colorants pour protéines dont la structure moléculaire est celle d'un fragment coumarine couplé à un hétérocycle ammonium quaternaire via une liaison vinyle ou polyvinyle, et qui sont capables de s'associer à des protéines de manière non covalente, non spécifique, à un faible pH, la forme associée présentant une émission de fluorescence significativement plus élevée que la forme non associée. Les colorants de l'invention peuvent par conséquent être utilisés comme étiquettes sélectives pour des protéines à faible pH, sans qu'il soit nécessaire d'enlever des composants étrangers du milieu dans lequel les protéines se trouvent avant la détection.

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