Counting compartment for biological investigations and a...

G - Physics – 02 – B

Patent

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Details

G02B 21/34 (2006.01) B01L 3/00 (2006.01) G01N 15/14 (2006.01)

Patent

CA 2212990

Counting compartment (1) having an inlet (5) and an outlet (6), and comprising a bottom plate (2) and a top plate (3), which are held at a distance from each other by material particles which have the same size and are situated at a distance from one another. The particles are surrounded by and embedded in a solid connecting layer formed by an adhesive. The connecting layer extends all round and up to the free end (11) of the outlet of the counting compartment. A fluent mixture of an adhesive mixed with electrostatically charged particles is used to form the counting compartment (1), the largest size of the material particles determining the depth of the counting compartment. The counting compartment is particularly suitable for counting cells in biological materials such as sperm and blood samples.

Compartiment à compter (1) comprenant une entrée (5) et une sortie (6), ainsi qu'une plaque inférieure (2) et une plaque supérieure (3) qui sont maintenues à une certaine distance l'une de l'autre par des particules de matière ayant la même dimension et étant situées à une certaine distance les unes des autres. Les particules sont entourées d'une couche de matériau de liaison solide formée par un adhésif et dans laquelle elles sont encastrées. La couche de liaison se prolonge tout autour et vers le haut jusqu'à l'extrémité libre (11) de la sortie du compartiment à compter. Ce dernier est formé d'un mélange fluide d'adhésif mélangé à des particules chargées électrostatiquement, sa profondeur étant déterminée par la plus grosse dimension des particules de matière. Le compartiment à compter (1) convient tout particulièrement au comptage de cellules dans les matériaux biologiques comme le sperme et les échantillons sanguins.

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