Coupling of antigens and antibodies to non-fixed erythrocytes

Details Coupling of antigens and antibodies to non-fixed erythrocytes Coupling of antigens and antibodies to non-fixed erythrocytes

G - Physics

01

N

G01N 33/555 (2006.01) G01N 33/76 (2006.01) G01N 33/80 (2006.01)

Patent

CA 2090392

1. In order to determine an analyte in an aqueous sample solution by agglutination of non-fixed erythrocytes, one proceeds according to the present invention in such a way that (a) erythrocytes are incubated with one or several antibodies or/and antibody derivatives which do not lead to premature agglutination whereby the antibodies or/and antibody derivatives are directed towards one or several antigens on tie erythrocyte membrane and whereby before or after incubation with the erythrocytes the antibodies or/and antibody derivatives are coupled to a first binding partner of a high affinity binding pair, (b) a second binding partner of the high affinity binding pair is incubated with the treated erythrocytes from (a) whereby the second binding partner is chosen such that it has more than one binding site per molecule for the first binding partner, (c) the first binding partner of the high affinity binding pair which is coupled with the analyte or/and an antigen-active determinant of the analyte is incubated with the treated erythrocytes from (b), (d) a sample solution which has been treated with an antibody or antibody derivative directed towards the analyte is incubated with the treated erythrocytes from (c) and (e) the absence or presence of the analyte in the sample solution is determined on the basis of the occurrence or absence of an agglutination reaction.

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