Coupling of lipopolysaccharide-derived carbohydrates onto...

Details Coupling of lipopolysaccharide-derived carbohydrates onto... Coupling of lipopolysaccharide-derived carbohydrates onto...

C - Chemistry, Metallurgy

07

H

C07H 3/06 (2006.01) C07B 61/00 (2006.01) C07H 3/04 (2006.01) C08B 37/00 (2006.01) C40B 40/04 (2006.01) G01N 33/543 (2006.01) G01N 33/569 (2006.01)

Patent

CA 2355292

The present invention provides a method for immobilising a polysaccharide (PS) to a solid surface, said polysaccharide having a keto-carboxy group (-C(=O-COOH) or a ketal or hemiketal group corresponding thereto, e.g. derived from KDO (2-keto-3-deoxy-D-mannooctonic acid), the method comprising the steps of: (a) forming a covalent bond between the carboxy group of the polysaccharide and a reporter molecule (RM). Comprising a recognition/substrate site (e.g. biotin or an anthraquinone); and (b) immobilising for diagnostic purposes, e.g. for the detection of bacterial infections from Gram-negative bacteria.

L'invention concerne une méthode permettant d'immobiliser un polysaccharide (PS) sur une surface solide. Ledit polysaccharide contient un groupe céto-carboxy (-C(=O)-COOH) ou un groupe cétal ou hémicétal correspondant, par exemple dérivé de KDO (acide 2-céto-3-désoxy-D-mannooctonique). Cette méthode consiste à : (a) former une liaison covalente entre le groupe carboxy du polysaccharide et une molécule rapporteur (RM) contenant un site de reconnaissance/substrat (par exemple, la biotine ou un anthraquinone); et (b) à immobiliser le conjugué ainsi obtenu à des fins diagnostiques, par exemple pour la détection d'infections aux bactéries Gram négatif.

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