Coupling of nucleic acids to solid support by photochemical...

Details Coupling of nucleic acids to solid support by photochemical... Coupling of nucleic acids to solid support by photochemical...

B - Operations, Transporting

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150/3, 252/37

B01J 20/24 (2006.01) C12Q 1/68 (2006.01) G01N 33/50 (2006.01) G01N 33/548 (2006.01)

Patent

CA 1215703

ABSTRACT A solid support capable of binding a nucleic acid thereto upon suitable irradiation, comprising (a) a solid substrate, (b) a photochemically reactive intercalator compound or other nucleic acid-binding ligands, and (c) divalent radical chemically linking the substrate and the ligand (b). Specifically, a hydroxy group-containing solid substrate such as nitrocellulose paper is linked via a bifunctional reagent such as cyanogen bromide or 1,4-butanediol- diglycidyl ether to an amino-substituted angelicin or psoralen or ethidium bromide which in turn is photo- chemically linked to a nucleic acid. The resulting immobilized nucleic acid probe is capable of hybridizing with complementary nucleic acid fragments and is thereby useful in diagnostic assays.

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