Covalent attachment of macromolecules on substrate surfaces

Details Covalent attachment of macromolecules on substrate surfaces Covalent attachment of macromolecules on substrate surfaces

C - Chemistry, Metallurgy

07

K

167/139, 402/573

C07K 17/08 (2006.01) C08F 8/02 (2006.01) C08F 8/30 (2006.01) C08F 12/08 (2006.01) G01N 33/543 (2006.01) G01N 33/547 (2006.01)

Patent

CA 1323729

23 COVALENT ATTACHMENT OF MACROMOLECULES ON SUBSTRATE SURFACES ABSTRACT OF THE DISCLOSURE Uncrosslinked polystyrene surfaces are derivatized under Friedel-Crafts conditions in a tetramethylenesulfone solvent using a substituted N-hydroymethyl acetamide derivatizing agent. The substituent is subject to nucleophilic substitution to allow for the binding of a wide variety of molecules, particularly specific binding pair members, to the surface. Clarity is maintained for monitoring observation of events on the surface through the polystyrene.

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