Covalent attachment of specific binding members to a solid...

Details Covalent attachment of specific binding members to a solid... Covalent attachment of specific binding members to a solid...

C - Chemistry, Metallurgy

07

K

530/17, 195/35,

C07K 17/00 (2006.01) C07K 17/06 (2006.01) C12N 11/00 (2006.01) C12N 11/06 (2006.01) G01N 33/543 (2006.01) G01N 33/547 (2006.01) G01N 33/569 (2006.01)

Patent

CA 2015938

The present invention provides novel methods for the covalent attachment of a specific binding member to a solid phase through the reaction of a thiolated solid phase and a specific binding member that is complexed to a heterobifunctional coupling agent. The resulting immobilized specific binding member can be used in diagnostic binding assays. The invention enables the production of immobilized specific binding members which have increased sensitivity, specificity and stability, while requiring less specific binding member be used.

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