Covalent oligonucleotide-horseradish peroxidase conjugate

Details Covalent oligonucleotide-horseradish peroxidase conjugate Covalent oligonucleotide-horseradish peroxidase conjugate

C - Chemistry, Metallurgy

12

N

195/1.12

C12N 9/96 (2006.01) C07H 21/00 (2006.01)

Patent

CA 1300532

Abstract of the Disclosure Covalent conjugates of oligonucleotide chains and horseradish peroxidase (HRP) are disclosed. The oligonucleotide chains are provided with one or more sulfhydryl moieties using selected sulfhydryl functionalizing reagents, and are coupled through the sulfhydryl groups to a complex of HRP and an activated ester such as mal-sac-HNSA. The functionalizing reagents are structured so as to introduce a spacer moiety which optimizes the distance between the oligonucleotide chain and the HRP label.

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