Covered sample well for use in nucleic acid assays and...

C - Chemistry – Metallurgy – 12 – Q

Patent

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C12Q 1/00 (2006.01) B01L 3/00 (2006.01) C12M 1/40 (2006.01) C12Q 1/68 (2006.01) G01N 33/50 (2006.01)

Patent

CA 2215561

An improved sample well is provided for carrying out a biological or chemical process, such as a nucleic acid assay or an immunoassay, on a liquid sample. The sample well includes a cap with a restricted aperture sufficient in size to receive the distal end of a disposable pipette tip. The pipette tip is initially inserted into the aperture to introduce a liquid sample into the sample well, and is allowed to remain within the aperture while the sample reacts with a reagent contained within the sample well. In this way, the pipette tip effectively closes off the sample well while the reaction is taking place, thereby reducing evaporative loss of the sample and avoiding cross-contamination with samples contained in adjacent sample wells. The sample wells may also be constructed of, or include therein, different materials which perform different functions in the biological or chemical process, thereby permitting assembly of different groups of sample wells for different assay formats.

Puits d'échantillons pour mettre en oeuvre une méthode biologique ou chimique, comme un essai d'acide nucléique ou un immuno-essai avec un échantillon liquide. Le puits comporte un capuchon avec une ouverture restreinte, suffisante pour recevoir l'extrémité distale du bout d'une pipette jetable. Le bout de la pipette est inséré initialement dans l'ouverture pour introduire un échantillon liquide dans le puits, et il demeure dans celle-ci pendant que l'échantillon réagit avec un réactif se trouvant dans le puits. De cette façon, le bout de la pipette bouche complètement le puits pendant la réaction, ce qui réduit les pertes d'échantillon par évaporation et empêche la contamination croisée avec les échantillons des puits voisins. Les puits peuvent également être constitués de matériaux divers qui jouent différents rôles dans le processus biologique ou chimique, ce qui permet de constituer des montages de groupes différents de puits, correspondant à des modes d'essai différents.

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