Creatinine iminohydrolase free from urease activity

Details Creatinine iminohydrolase free from urease activity Creatinine iminohydrolase free from urease activity

C - Chemistry, Metallurgy

12

Q

195/39, 150/15.2

C12Q 1/34 (2006.01) C12N 9/78 (2006.01)

Patent

CA 1139690

Abstract of The Disclosure A urease-free creatinine iminohydrolase-enzyme preparation obtained from an aerobic soil microorganism. The enzyme of the preparation prererably has a molecular weight of from about 250,000 to 300,000; a maximum activity at a pH between 7 and 8 as measured at 37°C; a Km of about 3 to 5 mM for creatinine as measured at 37°C, pH 7.5; and a specific activity for creatinine of at least about 1.0 unit per milligram of protein in the preparation as measured at 37°C, pH 7.5. The preferred enzyme preparation 18 derived from the aerobic soil microorganism ATCC 31,546. Assay methods, compositions, and elements containing the afore- mentioned urease-free creatinine iminohydrolase for the determination of creatinine in an aqueous liquid are also disclosed.

361327

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