Creation of variable length and sequence linker regions for...

Details Creation of variable length and sequence linker regions for... Creation of variable length and sequence linker regions for...

C - Chemistry, Metallurgy

12

N

C12N 15/10 (2006.01) C07K 14/705 (2006.01) C12N 15/62 (2006.01) C12N 15/83 (2006.01)

Patent

CA 2385609

Disclosed are methods and compositions for creating a DNA, RNA or protein molecule with two or more nucleic acid or polypeptide domains, respectively, joined by a linker region. These methods are used to generate random linker libraries of nucleic acids that encode dual-domain or multi-domain polypeptides. The linker regions are characterized by both length and sequence variability.

L'invention concerne des procédés et des compositions pour la création d'une molécule d'ADN, d'ARN ou de protéine, comprenant deux ou plusieurs domaines polypeptidiques ou nucléotidiques reliés par une région de liaison. Lesdites méthodes sont utilisées pour la génération de banques de segments de liaison aléatoires d'acides nucléiques codant pour des polypeptides à domaine double ou multiple. Lesdites régions de liaison se caractérisent par une longueur et une séquence variables.

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