Cross-linked polysaccharide and protein matrices and methods...

C - Chemistry – Metallurgy – 08 – B

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C08B 37/00 (2006.01) C08B 37/08 (2006.01) C08B 37/10 (2006.01) C12N 11/10 (2006.01) C07K 17/10 (2006.01)

Patent

CA 2620663

Methods for preparing cross-linked polysaccharide matrices by cross-linking one or more amino group containing polysaccharides or amino-functionalized polysaccharides with reducing sugars and/or reducing sugar derivatives. The resulting matrices may include polysaccharide matrices and composite cross- linked matrices including polysaccharides cross-linked with proteins and/or polypeptides. Additives and/or cells may also be included in or embedded within the matrices. Various different solvent systems and reducing sugar cross-linkers for performing the cross-linking are described. The resulting matrices exhibit various different physical, chemical and biological properties.

L'invention porte sur des procédés de préparation de matrices de polysaccharides réticulées consistant à réticuler un ou des polysaccharides contenant des groupes amino ou polysaccharides amino fonctionnalisés par des sucres réducteurs ou des dérivés de sucres réducteurs. Les matrices résultantes peuvent être des matrices de polysaccharides et des matrices composites réticulées dont des polysaccharides réticulés par des protéines et/ou des polypeptides. Des additifs et/ou des cellules peuvent être inclus ou enfouis dans les matrices. L'invention porte également sur différents systèmes de solvants et de sucres réducteurs réticulants assurant la réticulation. Les matrices résultantes présentent différentes propriétés physiques, chimiques et biologiques.

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