C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
195/1.14, 195/1.
C12N 15/66 (2006.01) C07K 14/635 (2006.01) C12N 15/10 (2006.01) C12N 15/70 (2006.01)
Patent
CA 1303527
3247-184 ABSTRACT OF THE DISCLOSURE A cross-over linker in single or double-stranded form comprising (a) a homology searching sequence homologous to a targeted base pair sequence of a cloning vehicle; and, (b) a ligatable end complementary to a ligatable end in or insertible into said cloning vehicle, may be used to induce intramolecular cross-over of genetic material and introduce, substitute or delete genetic material In a cloning vehicle. A particular embodiment of the cross-over linker (a "double-cross" linker) has first and second homology searching sequences and can be employed to remove a restriction enzyme site from a cloning vehicle thereby locking up or stabilizing the genetic architective of the cloning vehicle.
517966
Sung Wing L.
Tam Cherk S.
National Research Council Of Canada
Smart & Biggar
Sung Wing L.
Tam Cherk S.
LandOfFree
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