Cross priming amplification of target nucleic acids

Details Cross priming amplification of target nucleic acids Cross priming amplification of target nucleic acids

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01) C12P 19/34 (2006.01)

Patent

CA 2748822

Methods of amplifying target sequences by utilizing cross priming isothermal amplification are disclosed Methods of marking the amplification target sequence during the amplification reaction and rapid detection of the target sequence are also disclosed Reagent kits for rapid nucleic acid diagnosis and the nucleic acid detection of pathogenic microorganisms such as bacteria, viruses, as well as to diagnoses related to human genetic diseases are also disclosed

La présente invention concerne des procédés d'amplification de séquences cibles par amplification isotherme avec amorçage croisé. La présente invention concerne aussi des procédés de marquage de la séquence ciblée par l'amplification pendant la réaction d'amplification, ainsi que la détection rapide de la séquence cible. L'invention concerne également des kits de réactifs pour le diagnostic rapide des acides nucléiques et pour la détection des acides nucléiques de micro-organismes pathogènes tels que les bactéries et les virus, ainsi que pour le diagnostic de maladies génétiques chez l'homme.

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