Cross-species and multi-species display systems

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/63 (2006.01) C12N 1/19 (2006.01) C12N 15/79 (2006.01) C12N 15/81 (2006.01) C40B 40/02 (2006.01) C40B 50/06 (2006.01) G01N 33/53 (2006.01)

Patent

CA 2651111

The present invention provides expression vectors and helper display vectors which can be used in various combinations as vector sets for multi-species and cross-species display of polypeptides on the outer surface of prokaryotic genetic packages and/or eukaryotic host cells. The multi-species and cross-species display systems can be practiced using the vector sets of the invention without having to change or reengineer the display vectors. The display systems of the invention are particularly useful for displaying a genetically diverse repertoire or library of polypeptides on the surface of phage, bacterial host cells, yeast cells, and mammalian cells.

L'invention concerne des vecteurs d'expression et des vecteurs d'expression à la surface auxiliaires, qui peuvent être utilisés dans diverses combinaisons sous forme de jeux de vecteurs pour une expression multispécifique et hétérospécifique de polypeptides sur la surface externe de matériels génétiques procaryotes et/ou de cellules hôtes eucaryotes. Les systèmes d'expression multispécifique et hétérospécifique peuvent être mis en oeuvre à l'aide des jeux de vecteurs selon l'invention sans qu'il soit nécessaire de modifier ou de restructurer les vecteurs d'expression à la surface. Les systèmes d'expression à la surface selon l'invention sont particulièrement utiles pour l'expression d'un répertoire ou une banque génétiquement divers de polypeptides sur la surface de phages, de cellules hôtes bactériennes, de cellules de levure et de cellules de mammifères.

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