Crosslinked compositions comprising collagen and...

A - Human Necessities – 61 – L

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A61L 24/00 (2006.01) A61L 24/10 (2006.01) A61L 27/22 (2006.01) A61L 27/36 (2006.01)

Patent

CA 2533758

A composition comprising a collagen protein and demineralized bone matrix is described wherein the composition is chemically cross-linked with a carbodiimide such as N-(3-dimethylaminopropyl)-N-ethylcarbodiimide hydrochloride (EDC). The crosslinking reaction can be conducted in the presence of N-hydroxysuccinimide (NHS). The collagen can be in a porous matrix or scaffolding. The DBM can be in the form of particles dispersed in the collagen. A method of making the composition is also described wherein a collagen slurry is cast into the desired shape, freeze dried to form a porous scaffolding and infiltrated with a solution comprising the cross-linking agent. The composition can be used as an implant for tissue (e.g., soft tissue or bone) engineering.

L'invention concerne une composition renfermant une protéine collagène et une matrice osseuse déminéralisée (DBM), la composition étant chimiquement réticulée avec un carbodiimide, tel que de l'hydrochlorure de N-(3-diméthylaminopropyl)-N-éthylcarbodiimide (EDC). La réaction de réticulation peut être effectuée en présence de N-hydroxysuccinimide (NHS). Le collagène peut être dans une matrice ou échafaudage poreux. La matrice DBM peut se présenter sous la forme de particules dispersées dans le collagène. L'invention concerne enfin un procédé de préparation de la composition dans lequel une suspension de collagène est moulée dans la forme souhaitée, lyophilisée de manière à former un échafaudage poreux et infiltrée d'une solution renfermant l'agent de réticulation. La composition peut être utilisée comme implant pour l'ingénierie tissulaire (par exemple, partie molle ou os).

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