Crosstalk inhibitors and their uses

Details Crosstalk inhibitors and their uses Crosstalk inhibitors and their uses

C - Chemistry, Metallurgy

07

K

C07K 14/765 (2006.01) C07D 207/416 (2006.01) C07H 21/00 (2006.01) C07K 16/00 (2006.01) C08K 5/3415 (2006.01) C12N 9/16 (2006.01) C12Q 1/68 (2006.01) C12Q 1/70 (2006.01) G01N 33/53 (2006.01) G01N 33/543 (2006.01) G01N 33/74 (2006.01) G01N 33/94 (2006.01)

Patent

CA 2107894

Assay for detecting the amount or presence of target ligand in a sample. The assay includes a ligand analogue conjugate having a linkage site and a binding site, a ligand receptor, and a sample. The assay includes the steps of providing at least one crosstalk inhibitor. This inhibitor, under assay conditions, competes with the linkage site of the ligand analogue conjugate for binding to the ligand receptor, and does not compete with the binding site of the ligand analogue conjugate for binding to the li- gand receptor. In the invention, the assay is performed for the target ligandin the presence of a sufficient amount of the crosstalk inhibitorto reduce the amount of binding of the linkage site of the ligandanalogue conjugate to the ligand receptor. The invention also features a method for identifying crosstalk inhibitors, and the crosstalk inhibitors themselves.

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