Cryopreservation medium for primate embryo stem cells and...

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 5/02 (2006.01) C12N 5/0735 (2010.01) A01N 1/02 (2006.01) C12N 1/04 (2006.01)

Patent

CA 2474968

It is intended to provide a liquid for frozen storage and a frozen storage method whereby primate embryo stem cells can be conveniently stored in a frozen state at a high survival rate. A liquid for frozen storage of primate embryo stem cells characterized by containing a frozen protecting agent at a concentration of from 12% (W/V) to 50% (W/V). A frozen storage method for primate embryo stem cells which comprises the step of suspending primate embryo stem cells in the liquid for frozen storage as described above, the cooling step of freezing the liquid for frozen storage containing the primate embryo stem cells suspended therein by cooling to -80~C at a rate of 0.5 to 10~C per minute, and the storage step of storing the thus frozen liquid for frozen storage containing the primate embryo stem cells suspended therein at - 80~C or lower. Thus, primate embryo stem cells can be conveniently stored in a frozen state at a high survival rate.

L'invention concerne un liquide de stockage par congélation et un procédé de stockage par congélation permettant un stockage adéquat à l'état congelé des cellules souches embryonnaires de primates avec un taux de survie élevé. Le liquide de stockage par congélation servant au stockage de cellules souches embryonnaires de primates est caractérisé en ce qu'il contient un agent protecteur de congélation à raison d'une concentration comprise entre 12 % (W/V) et 50 % (W/V). Le procédé de stockage à l'état congelé de cellules souches comprend une étape consistant à mettre les cellules souches embryonnaires de primates en suspension dans le liquide de stockage décrit, une étape de refroidissement consistant à congeler le liquide de stockage contenant les cellules souches embryonnaires de primate en suspension en le refroidissant à 80 ·C à une vitesse de 0,5 à 10 ·C par minute, et une étape de stockage consistant à stocker le liquide de stockage ainsi congelé contenant les cellules souches embryonnaires de primate en suspension, à une température égale ou inférieure à -80 ·C. Ce procédé permet de stocker de manière pratique les cellules souches embryonnaires de primate à l'état congelé, avec un taux de survie important.

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