Cryopreservation of cultured tissue equivalents

Details Cryopreservation of cultured tissue equivalents Cryopreservation of cultured tissue equivalents

A - Human Necessities

01

N

A01N 1/02 (2006.01) B65D 55/06 (2006.01) B65D 77/20 (2006.01) C12N 5/08 (2006.01) B65D 85/50 (2006.01)

Patent

CA 2182814

This invention is directed to cryopreservation of cultured tissue equivalents made by in vivo technology. The invention involves immersing a cultured tissue equivalent in a cooled cryoprotectant solution, agitating the cryoprotectant solution and the immersed cultured tissue to achieve effective penetration of the cryoprotectant solution into the cultured tissue equivalent, and then freezing the cultured tissue at a high freezing rate. The cryopreserved cultured tissue equivalent may be stored for indefinite periods of time prior to use. The cultured tissue equivalent is an in vivo model of the equivalent human tissue, which, when retrieved from storage, can be used for transplantation or implantation in vivo or for screening compounds in vivo.

Cryopréservation d'équivalents tissulaires cultivés qui sont conçus selon la technologie in vivo. Cette invention comprend : l'immersion d'un équivalent tissulaire cultivé dans une solution cryoprotectrice refroidie; l'agitation de cette solution et du tissu cultivé immergé pour permettre une pénétration efficace de la solution dans l'équivalent tissulaire; la congélation du tissu cultivé à un taux élevé de congélation. L'équivalent tissulaire cryopréservé peut être entreposé pour une durée indéterminée avant son utilisation. Il constitue un modèle in vivo du tissu humain équivalent qui, au terme de l'entreposage, peut servir à la transplantation ou à l'implantation in vivo ou encore au tri de composés in vivo.

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