Cryopreservation of sperm

A - Human Necessities – 01 – N

Patent

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A01N 1/00 (2006.01) A01N 1/02 (2006.01)

Patent

CA 2417595

The invention features methods of cryopreserving sperm. The methods include providing a sample of sperm; cooling the sperm to a first temperature; maintaining the sperm sample at the first temperature; cooling the sperm to a second temperature; maintaining the sperm at the second temperature; and storing the sperm at a third temperature. These methods can be used to cryopreserve sperm from mammals, e.g. transgenic mammals and can be to preserve sperm for subsequent artificial insemination or in vitro fertilization.

L'invention concerne des procédés de cryopréservation du sperme. Ces procédés consistent notamment à: fournir un échantillon de sperme; le refroidir à une première température; le conserver à cette première température; le refroidir à une deuxième température; le conserver à cette deuxième température; et le stocker à une troisième température. On peut utiliser ces procédés pour la cryopréservation du sperme provenant de mammifères, par exemple de mammifères transgéniques, et pour sa conservation en vue d'inséminations artificielles futures ou de fécondations in vitro.

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