C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/31 (2006.01) A61K 39/00 (2006.01) A61K 39/395 (2006.01) C07K 14/40 (2006.01) C07K 16/14 (2006.01) C12Q 1/68 (2006.01) G01N 33/569 (2006.01)
Patent
CA 2237134
We have recently described the production of monoclonal antibodies (MAbs) directed against surface antigens of the Candida albicans yeast and mycelial forms, respectively. The mycelial surface antigen recognized by MAb 4E1 was shown to be present predominantly in the terminal third of the hyphal structures. We have now cloned the corresponding gene (CSA1) by MAb 4E1-coated magnetic beads sorting of S. cerevisiae transformants expressing a Candida albicans genomic library. The strategy is both highly selective and sensitive and provides an additional genetic tool to facilitate cloning of Candida genes encoding surface proteins. CSA1 is an intronless single copy gene in Candida and whereas RNA transcripts can be detected in exponentially growing yeast cells, gene expression is strongly increased upon induction of the mycelial growth phase. The deduced amino acid sequence of Csa1p (1203-residues) shares many features with other cell wall proteins including the presence of repetitive motifs and domains. Most strikingly, a Cys-rich hydrophobic domain of 102 amino acids that is repeated five times in the protein shows significant sequence similarity with a major T-cell immunoreactive antigen (Ag2) from another opportunistic fungal human pathogen; Coccidioides immitis. This suggests that Csa1-like proteins may confer unique properties to the cell wall of fungal pathogens.
Bourbonnais Yves
Deslauriers Noella
Bourbonnais Yves
Deslauriers Noella
Swabey Ogilvy Renault
Universite Laval
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