Cultivation apparatus for cell, tissue or both, and...

C - Chemistry – Metallurgy – 12 – M

Patent

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Details

C12M 3/00 (2006.01) C12N 5/07 (2010.01) C12N 5/02 (2006.01)

Patent

CA 2658235

An apparatus for, and method of, cultivation of a culture of cell line, tissue, etc. There is provided an apparatus comprising incubator part (4) for accommodating of a culture and, passing through the incubator part within and without, lever (70) capable of circular arc motion around the center of a supporting point set in a wall part of the incubator part or in the vicinity thereof, which apparatus is so structured as to impart displacement to the culture through manipulation of the lever. Bending force can be applied to the culture of cell line, tissue, etc. (cell constitution (6)), so that without any increase or decrease of culture fluid (48) within the incubator part, namely, without any increase or decrease of pressure on the culture fluid, displacement required for cultivation can be imparted to the culture within the incubator part. By curving, there are brought about continuous compression and extension in the direction of thickness from a concave portion to convex portion thereof.

L'invention a pour objet un appareil et un procédé pour la culture d'une lignée cellulaire, de tissus, etc. L'appareil comprend une partie incubatrice (4) destinée à recevoir la culture et, traversant la partie incubatrice (4) à l'intérieur et à l'extérieur, un levier (70) pouvant effectuer un mouvement en arc de cercle autour du centre d'un point d'appui placé dans une paroi de la partie incubatrice (4) ou à proximité, l'appareil étant conçu de manière à imprimer un déplacement à la culture par le maniement du levier (70). Une force de flexion peut être appliquée à la culture de lignée cellulaire, tissu, etc. (constitution cellulaire (6)) afin de lui imprimer le déplacement requis pour la culture à l'intérieur de la partie incubatrice (4) sans aucune augmentation ou diminution du liquide de culture (48), à savoir sans aucune augmentation ou diminution de pression du liquide de culture. La flexion produit une compression continue et une extension dans le sens de l'épaisseur d'une partie concave à une partie convexe.

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